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TMB was calculated as the ratio between the number of mutations and the dimensions of the sequenced regions (35.7 Mb). For those sufferers with available RNAseq, quartiles of FPKM have been calculated. Mutations recognized by WES evaluation were considered as “expressed mutations” if a minimum of one of many gene counter algorithms was expressed in 3rd or 4th quartile. Once mutations had been filtered, 29-mer amino acid sequences, containing the mutated residue at place 15, have been designed.

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